abstract：:An alternative 16S rRNA-targeted oligonucleotide probe specific for Archaea was developed and used for detection of methanogens in anaerobic reactors. The designed probe was checked for its specificity by computer-aided comparative sequence analysis. For in situ application, optimal stringency conditions were adjusted...

journal_title：Journal of microbiological methods

authors： Jupraputtasri W,Boonapatcharoen N,Cheevadhanarak S,Chaiprasert P,Tanticharoen M,Techkarnjanaruk S

更新日期：2005-04-01 00:00:00

abstract：:Brucella is a Gram-negative facultative bacterium that persists intracellularly in macrophages. However, the intracellular survival mechanisms used by Brucella are not fully understood. Isolation of Brucella RNA from infected macrophages has been challenging, and the inability to isolate sufficient Brucella RNA from i...

journal_title：Journal of microbiological methods

authors： Covert J,Eskra L,Splitter G

更新日期：2005-03-01 00:00:00

abstract：:Thermophilic strains of Geobacillus, Anoxybacillus and Bacillus that are able to grow at 55 degrees C and above are recognized as commonly occurring contaminants during the production of milk powders. In particular, Anoxybacillus flavithermus strain C and Bacillus licheniformis strain F are often the most prevalent. W...

journal_title：Journal of microbiological methods

authors： Rueckert A,Ronimus RS,Morgan HW

更新日期：2005-02-01 00:00:00

abstract：:The development of rapid methods for detection of viable Listeria monocytogenes is crucial to prevent listeriosis and product recalls. While immunomagnetic separation has been used for isolating Listeria spp., lack of specificity and pathogenicity determination render this method unsatisfactory. A two-step method usin...

journal_title：Journal of microbiological methods

authors： Gray KM,Bhunia AK

更新日期：2005-02-01 00:00:00

abstract：:Ureaplasma parvum and Ureaplasma urealyticum are recently recognized species of the genus Ureaplasma. In humans, Ureaplasma spp. can be found on mucosal surfaces, primarily in the respiratory and urogenital tracts. They have been implicated in various human diseases such as nongonococcal urethritis, intrauterine infec...

journal_title：Journal of microbiological methods

authors： Mallard K,Schopfer K,Bodmer T

更新日期：2005-01-01 00:00:00

abstract：:Despite advances in antifungal prophylaxis and therapy, morbidity and mortality incurred by yeasts remain a significant burden. As pathogenic yeast species vary in their susceptibilities to antifungal agents, clinical microbiology laboratories face an important challenge to identify them rapidly and accurately. Althou...

journal_title：Journal of microbiological methods

authors： Plachý R,Hamal P,Raclavský V

更新日期：2005-01-01 00:00:00

abstract：:The spores and conidia of most fungi have very thick and resistant cell walls that severely impede the staining with fluorescent dyes to allow epifluorescence microscopy to be employed for their direct detection and quantification in natural habitats. In this study, oxidation by sodium hypochlorite and microwave irrad...

journal_title：Journal of microbiological methods

authors： Prigione V,Filipello Marchisio V

更新日期：2004-12-01 00:00:00

abstract：:In this study, enrichment procedures and two recovery methods, a membrane surface adhesion technique and an immunomagnetic separation (IMS), were compared for use in conjunction with a multiplex polymerase chain reaction (PCR) method with a view to describing a fast (24 h) and economical test for detection of Escheric...

journal_title：Journal of microbiological methods

authors： Fitzmaurice J,Duffy G,Kilbride B,Sheridan JJ,Carroll C,Maher M

更新日期：2004-11-01 00:00:00

abstract：:Microscopic discrimination between extracellular and invasive, intracellular bacteria is a valuable technique in microbiology and immunology. We describe a novel fluorescence staining protocol, called FITC-biotin-avidin (FBA) staining, which allows the differentiation between extracellular and intracellular bacteria a...

journal_title：Journal of microbiological methods

authors： Agerer F,Waeckerle S,Hauck CR

更新日期：2004-10-01 00:00:00

abstract：:In various recent experiments with respect to protein secretion by L. pneumophila, protein detection based on the cMyc-tag seemed to be problematic. For one specific protein, we studied the reason for this problem in more detail by comparing protein expression in the presence and absence of the cMyc-tag. We demonstrat...

journal_title：Journal of microbiological methods

authors： De Buck E,Lebeau I,Van Mellaert L,Geukens N,Anné J,Lammertyn E

更新日期：2004-10-01 00:00:00

abstract：:This study describes a new capillary-type microplate multiassay for characterization of protozoal chemotactic responses, allowing up to 32 assays to be run simultaneously. We used the new multiassay to evaluate the chemoattractant activity of turbot blood components and turbot cells for the facultative parasite Philas...

journal_title：Journal of microbiological methods

authors： Paramá A,Iglesias R,Alvarez MF,Sanmartín ML,Leiro J

更新日期：2004-09-01 00:00:00

abstract：:The use of a minimal lactose enrichment broth (MLB) in conjunction with immunomagnetic electrochemiluminescence detection (IM-ECL) was evaluated for the estimation of viable Escherichia coli O157 populations in surface water samples. In principle, E. coli O157 populations (C(initial E. coli O157)) can be derived from ...

journal_title：Journal of microbiological methods

authors： Shelton DR,Higgins JA,Van Kessel JA,Pachepsky YA,Belt K,Karns JS

更新日期：2004-08-01 00:00:00

abstract：:Ruthenium red is a polycationic stain used to visualize acid polysaccharides on the outer surface of cells. Ruthenium red staining followed by electron microscopic analysis was used to demonstrate the presence of an external glycoprotein layer surrounding the spore of both Bacillus anthracis and Bacillus subtilis. Thi...

journal_title：Journal of microbiological methods

authors： Waller LN,Fox N,Fox KF,Fox A,Price RL

更新日期：2004-07-01 00:00:00

abstract：:Recently developed 13CO2 pulse labelling and stable isotope probing (SIP) methods offer the potential to track 13C-labelled plant photosynthate into phylogenetic groups of microbial taxa in the rhizosphere, permitting an examination of the link between soil microbial diversity and carbon flow in situ. We tested the fe...

journal_title：Journal of microbiological methods

authors： Griffiths RI,Manefield M,Ostle N,McNamara N,O'Donnell AG,Bailey MJ,Whiteley AS

更新日期：2004-07-01 00:00:00

abstract：:A flexible, extendable tool for the optimization of (micro)biological processes and protocols using evolutionary algorithms was developed. It has been tested using three different theoretical optimization problems: 2 two-dimensional problems, one with three maxima and one with five maxima and a river autopurification ...

journal_title：Journal of microbiological methods

authors： Sys K,Boon N,Verstraete W

更新日期：2004-06-01 00:00:00

abstract：:Complementation of chromosomal mutations in trans can introduce artifacts due to the number of episomal copies of the gene in question. One solution is to study the gene expressed at a single ectopic site in cis. We have designed and constructed a vector that allows homologous recombination into a gene encoding a fram...

journal_title：Journal of microbiological methods

authors： Daines DA,Smith AL

更新日期：2004-06-01 00:00:00

abstract：:A 16S rRNA gene-based fingerprinting method was developed for the identification of Azotobacteraceae and tested onto 48 soil isolates and 28 reference strains belonging to the free-living nitrogen-fixing bacterial group and to the most common species found in soil samples. According to this method, the 16S rRNA gene w...

journal_title：Journal of microbiological methods

authors： Aquilanti L,Mannazzu I,Papa R,Cavalca L,Clementi F

更新日期：2004-05-01 00:00:00

abstract：:Supplemented MacConkey agar with L-rhamnose and D-arabitol distinguishes pathogenic from environmental strains of Yersinia enterocolitica recovered from swine production wastes. This medium has a sensitivity of 100% and a specificity of 97.4%. ...

journal_title：Journal of microbiological methods

authors： Shehee MW,Sobsey MD

更新日期：2004-05-01 00:00:00

abstract：:Specific molecular determination and classification of ammonia-oxidizing bacteria have relied on the use of conventional markers such as 16S rDNA. However, this gene does not satisfactorily provide a wide vision of all phylogenetic lineages. Despite the initial expectations, the use of functional genes as for example ...

journal_title：Journal of microbiological methods

authors： Calvó L,Garcia-Gil LJ

更新日期：2004-04-01 00:00:00

abstract：:Bacillus anthracis, the causative agent of the human disease anthrax, Bacillus cereus, a food-borne pathogen capable of causing human illness, and Bacillus thuringiensis, a well-characterized insecticidal toxin producer, all cluster together within a very tight clade (B. cereus group) phylogenetically and are indistin...

journal_title：Journal of microbiological methods

authors： La Duc MT,Satomi M,Agata N,Venkateswaran K

更新日期：2004-03-01 00:00:00

abstract：:A simple and sensitive method based on the use of colloidal chitin stained with Remazol Brilliant Blue R (RBB) is proposed to evaluate chitinase activity. If this colloidal-stained substrate is included as a carbon source in a liquid medium, this technique allows the selection or the comparison of chitinolytic microor...

journal_title：Journal of microbiological methods

authors： Gómez Ramírez M,Rojas Avelizapa LI,Rojas Avelizapa NG,Cruz Camarillo R

更新日期：2004-02-01 00:00:00

abstract：:We have here applied high-throughput amplified fragment length polymorphism (htAFLP) analysis to strains belonging to the five classical species of the Mycobacterium tuberculosis complex. Using 20 strains, three enzyme combinations and eight selective amplification primer pairs, 24 AFLP reactions were performed per st...

journal_title：Journal of microbiological methods

authors： van den Braak N,Simons G,Gorkink R,Reijans M,Eadie K,Kremers K,van Soolingen D,Savelkoul P,Verbrugh H,van Belkum A

更新日期：2004-01-01 00:00:00

abstract：:Efficient selection of new silage inoculant strains from a collection of over 10,000 isolates of lactic acid bacteria (LAB) requires excellent strain discrimination. Toward that end, we constructed a GelCompar II database of DNA fingerprint patterns of ethidium bromide-stained EcoRI fragments of total LAB DNA separate...

journal_title：Journal of microbiological methods

authors： Chan RK,Wortman CR,Smiley BK,Hendrick CA

更新日期：2003-12-01 00:00:00

abstract：:Cryptosporidium is a zoonotic coccidian parasite associated with diarrhea, and the disinfectant-resistant oocysts are threats to public health even in industrialized countries. In order to make an accurate assessment of the risk to public health, a detection method that has a high recovery rate of oocysts in water is ...

journal_title：Journal of microbiological methods

authors： Inoue M,Rai SK,Oda T,Kimura K,Nakanishi M,Hotta H,Uga S

更新日期：2003-12-01 00:00:00

abstract：:As part of a major European research project, a diagnostic PCR assay, including an internal amplification control, was developed and validated in a collaborative trial for the detection of Escherichia coli O157. The assay is based on amplification of sequences of the rfbE O157 gene. The collaborative trial, including ...

journal_title：Journal of microbiological methods

authors： Abdulmawjood A,Bülte M,Cook N,Roth S,Schönenbrücher H,Hoorfar J

更新日期：2003-12-01 00:00:00

abstract：:Flow cytometry was used to characterize bacterioplankton from two tropical environments in Brazil: the eutrophic Guanabara Bay and the oligotrophic southwest Atlantic Ocean. Bacterial abundance was evaluated by flow cytometry, and cells were stained with SYTO 13, allowing demonstration of differences in nucleic acid c...

journal_title：Journal of microbiological methods

authors： Andrade L,Gonzalez AM,Araujo FV,Paranhos R

更新日期：2003-12-01 00:00:00

abstract：:A method to detect and enumerate bacterial colonies grown on membrane filters (MF) was described. The colonies were stained with an ethanolic solution of 0.1% Amido black 10B. The procedure yielded the rapid detection of colonies as compared to a conventional plate counting method. ...

journal_title：Journal of microbiological methods

authors： Inatomi K

更新日期：2003-11-01 00:00:00

abstract：:For routine mass screening, the use of microarrays is hampered because one chip can only analyze one sample. 16S rRNA gene PCR products of several bacterial strains or mixtures thereof were consecutively loaded on a single electronic microarray and successfully analyzed using probes specific for the bacterial strains....

journal_title：Journal of microbiological methods

authors： Zimmermann K,Eiter T,Scheiflinger F

更新日期：2003-11-01 00:00:00

abstract：:A rapid sonication method for lysis of Gram-positive bacteria was evaluated for use in combination with quantitative real-time polymerase chain reaction (PCR) analyses for detection. Other criteria used for evaluation of lysis were microscopic cell count, colony forming units (cfu), optical density at 600 nm and total...

journal_title：Journal of microbiological methods

authors： Fykse EM,Olsen JS,Skogan G

更新日期：2003-10-01 00:00:00

abstract：:A rapid, simple and sensitive spectrofluorimetric technique was developed for monitoring total lipids in hyphae of the riboflavin-overproducing fungus Eremothecium ashbyii using the fluorescent probe Nile blue in an aqueous system, avoiding the interference due to autofluorescent riboflavin. The existing methodologies...

journal_title：Journal of microbiological methods

authors： Vijayalakshmi S,Karthika TN,Mishra AK,Chandra TS

更新日期：2003-10-01 00:00:00

abstract：:Two novel modifications of continuous culture with gradual change of dilution rate (A-stat): D-stat and auxo-accelerostat were evaluated in the studies of the effect of changing individual environmental parameters (T, pH, pO(2), substrate concentration, etc.) on growth characteristics of different microorganisms. Comm...

journal_title：Journal of microbiological methods

authors： Kasemets K,Drews M,Nisamedtinov I,Adamberg K,Paalme T

更新日期：2003-10-01 00:00:00

abstract：:A method for rapidly and selectively isolating Salmonellae from buffer solutions and concentrating the bacteria by a factor of approximately 500 was developed. Anti-Salmonellae antibody was covalently linked to 40 microm polyacrylamide beads to prepare a solid phase with affinity for the bacteria. The beads were packe...

journal_title：Journal of microbiological methods

authors： Brewster JD

更新日期：2003-10-01 00:00:00

abstract：:Expressed sequence tags (ESTs) from fungal-infected plant tissues are composed of a mixture of plant and fungal sequences. Using freely available software and tools, a novel procedure is described for distinguishing plant and fungal DNA sequences. Although the GenBank non-redundant (NR) database is larger and therefor...

journal_title：Journal of microbiological methods

authors： Hsiang T,Goodwin PH

更新日期：2003-09-01 00:00:00

abstract：:We have developed a software tool, GenomeComp, for summarizing, parsing and visualizing the genome sequences comparison results derived from voluminous BLAST textual output. With GenomeComp, the variation between genomes can be easily highlighted, such as repeat regions, insertions, deletions and rearrangements of gen...

journal_title：Journal of microbiological methods

authors： Yang J,Wang J,Yao ZJ,Jin Q,Shen Y,Chen R

更新日期：2003-09-01 00:00:00

abstract：:The spore is the form released in a bioterrorism attack. There is a real need for definition of new targets for Bacillus anthracis that might be incorporated into emerging biodetection technologies. Particularly of interest are macromolecules found in B. anthracis that are (1) spore-specific, (2) readily accessible on...

journal_title：Journal of microbiological methods

authors： Fox A,Stewart GC,Waller LN,Fox KF,Harley WM,Price RL

更新日期：2003-08-01 00:00:00

abstract：:Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified ribosomal RNA gene amplicons was used to study the stool microbiota of hospitalized patients and to examine the effect of antibiotic therapy. For one patient, 16 anaerobic species identified by random cloning and sequencing of PCR-amplified rRNA genes fro...

journal_title：Journal of microbiological methods

authors： Donskey CJ,Hujer AM,Das SM,Pultz NJ,Bonomo RA,Rice LB

更新日期：2003-08-01 00:00:00

abstract：:We quantified both DNA and humic acid concentrations during the extraction and purification of DNA from compost. The DNA extraction method consisted of bead-beating with SDS for cell lysis, poly(ethylene glycol)-8000 precipitation for preliminary DNA purification, and chromatography on a 10-ml Sephadex G-200 column fo...

journal_title：Journal of microbiological methods

authors： Howeler M,Ghiorse WC,Walker LP

更新日期：2003-07-01 00:00:00

abstract：:Terminal Restriction Fragment (TRF) pattern analysis has become a widely used and informative tool for studying microbial communities. Variation between sequence-determined or true TRF length and observed TRF length (TRF drift) has been previously reported and can significantly affect identification of bacterial speci...

journal_title：Journal of microbiological methods

authors： Kaplan CW,Kitts CL

更新日期：2003-07-01 00:00:00

abstract：:Fluorescence in situ hybridization has been used for the identification and analysis of populations of the dinoflagellate Symbiodinium that lives symbiotically in marine invertebrates. Conditions for in situ hybridization of Symbiodinium were optimized and used to identify the clade to which the isolate belongs using ...

journal_title：Journal of microbiological methods

authors： Yokouchi H,Takeyama H,Miyashita H,Maruyama T,Matsunaga T

更新日期：2003-06-01 00:00:00

abstract：:Characterization of clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and representatives of three MRSA clones from other hospitals was performed by arbitrarily primed polymerase chain reaction (AP-PCR) and antibiotic susceptibility patterns. All isolates were mecA-positive and two main antibioty...

journal_title：Journal of microbiological methods

authors： Bartzavali-Louki C,Dimitracopoulos G,Spiliopoulou I

更新日期：2003-06-01 00:00:00